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W!N isE: daN tuBven with thegiteal editionn.The acevaluatioprocedure or ote“ (below).der ”NunsoaleSeCuvette TestLCK 554PrincipleDetermination of 5-day biochemical oxygen demand with inhibitionof nitrification by 5 mg/l allylthiourea. The dissolved oxygen isanalysed in an alkaline solution with a pyrocatechol derivative in the2 presence of Fe , under which conditions a red dye is formed.The transfer pipettes should be discarded after use. Thebeakers should be cleaned thoroughly with hot tap waterafter use, or, if strongly soiled, with a suitable cleansingagent.GBRange of ApplicationSurface water, low-load outflows of municipal or industrial sewagetreatment plants with biological purification stage without additionalinoculation.For special attentionSamples of surface water that contain no municipal wastewater are inoculated by means of a simplified procedure priorto the analysis (see application).LCK 554BOD5Storage InformationThe test reagents are stable at 2 to 8 C up to the expiry date given on the package.Safety AdviceOn grounds of quality and reliability, the analysis should be carriedout only with original Dr. Lange accessories.InterferencesPeroxide compounds, powerful oxidizing agents, high chlorineconcentrations and powerful reducing agents cause high-bias orlow-bias results and interfere with the biochemical oxidation over5 days.–2 Nitrite (NO2 ) and Iron (Fe ) can interfere with the reaction if presentin concentrations of at least 1 mg/l in the original sample.The COD content should not exceed 25 mg/l in the original sampleor 50 mg/l in the 1 : 2 dilution.Samples with a high particulate content interfere with the determination – if necessary, carry out the analysis with the supernatant liquidafter homogenizing and allowing the sediment to settle out.Preserved or frozen samples can only be analysed with this procedurewith the application (simplified inoculation procedure).The measurement results must be subjected to plausibility checks(dilute and/or spike the water sample). This can be done withLCK 554 BOD 5 by means of a multiple determination.DosiCap Zip with reagent tablets andglass beads (LCK 554 A) contains:Lithium hydroxideR: 36/38 S: 26-37/39Removal of InterferencesSamples that contain algae must be filtered before the analysis(1.2 µm filter; membrane filtration set LCW 904; to avoid high-biasresults, rinse the filter with distilled water before use).XiR: (36/38) Irritating to eyes and skin.S: (26) In case of contact with eyes, rinse immediately with plenty ofwater and seek medical advice. (37/39) Wear suitable gloves andeye/face protection.CADAS 100 (LPG 185 / LPG 210)If this test is not already stored in your instrument please ask yourDr. Lange Agency for programming instructions.NoteThe change indicated by the new edition date and the new colourof the working procedure is related to the introduction of the dualfunction DosiCap Zip, which simplifies the addition of the tabletsand glass beads and also serves as a sealing cap.pH/TemperatureThe pH of the water sample must be between pH 4 and pH 10.The temperature of the water sample and the dilution water must bebetween 18 and 24 C.AE 554 E / Druckfarbe blau / 1

Procedure ILCK 554Procedure IIApplies to all types of photometerApplies to all types of photometerEdition 98/04BOD5Sample PreparationPlease take note of the information under the heading”For special attention“.1. Original sampleIntroduce the magnetic stirring bar into a 100 ml beaker.Into the same beaker pipetteWastewater sample40 mlPlace the beaker on the Dr. Lange magnetic stirrer and stir for5 min at 500 – 750 rpm. This oxygenates the wastewater sample.2. Diluted sampleIntroduce the magnetic stirring bar into a 100 ml beaker.Into the same beaker pipetteWastewater sample20 mlchlorine-free drinking water (BOD5 0.5 mg/l)20 mlPlace the beaker on the Dr. Lange magnetic stirrer and stir for5 min at 500 – 750 rpm. This oxygenates the wastewater sample.The dilution factor must be taken into account when theBOD 5 content is calculated (measurement result x 2).Two sample cuvettes are needed for each wastewater sample.The sample cuvette 1 is measured directly and thesample cuvette 2 is measured after 5 days.It is advisable to label the cuvettes:e.g. A1 direct measurementA5 measurement after 5 daysA transfer pipette is used to fill the two sample cuvettes up tothe brim in sequence with the prepared wastewater sample.Ensure that the sample cuvette 2 (measurement after 5 days)is free of air bubbles and seal it immediately. Leave it for5 days at 20 C in the dark in a temperature-controlled cabinetor LT 20 dry thermostat.Data tableLCK 554LP2W98/04BOD5 λ: 620 nm Pro.: 1 F1 0 F2 9.858 K 0ISIS 6000/900098/04BOD5 λ: 610 nm Pro.: 1 F1 0 F2 8.014 K 0CADAS 100 / LPG 18598/04BOD5 λ: 620 nm F1 9.63 F2 0CADAS 100 / LPG 210BOD5 λ: 620 nm F1 9.63 K 098/04Edition 99/02BOD5Procedure II: direct and after 5 days1. Sample cuvette1 (direct measurement) [see diagram A – I]Place the funnel on the filled sample cuvette 1. Carefully pullthe aluminium foil off the DosiCap Zip and pour the contents(tablets and glass beads) through the funnel into the samplecuvette 1. Remove the funnel and immediately seal thesample cuvette 1 with DosiCap Zip, taking care that the cuvettecontains no air bubbles. NB: If the liquid meniscus fallsbelow the cuvette opening when the funnel is removed, makeup the volume by adding 2 to 4 glass beads. Repeatedly invertthe sample cuvette 1 for 3 min until the reagent tablets arecompletely dissolved. Wait 3 min, then thoroughly clean theoutside of the cuvette again and evaluate immediate.Record the result!2. Sample cuvette 2 (measurement after 5 days) [see diagram A – I]Open sample cuvette 2, which has been left to stand for 5 days,and place the funnel on it. Carefully pull the aluminium foiloff the DosiCap Zip and pour the contents (tablets and glassbeads) through the funnel into the sample cuvette 2. Removethe funnel and immediately seal the sample cuvette 2 withDosiCap Zip, taking care that the cuvette contains no air bubbles.NB: If the liquid meniscus falls below the cuvette opening whenthe funnel is removed, make up the volume by adding 2 to 4glass beads. Repeatedly invert the sample cuvette 2 for 3 minuntil the reagent tablets are completely dissolved. Wait 3 min,then thoroughly clean the outside of the cuvette again andevaluate immediate.Record the result!Application98/04BOD5 F1 0 F2 10.41 K 0CADAS 30/30S/50/50SLCK 554LCK 554Applies to all types of photometerBOD5AusgabeEdition 99/0298/04Simplified inoculation procedure for surface water that isonly minimally microbiologically contaminated. Theprocedure should be used for surface water that containsno municipal waste water.AccessoriesLZC 555 BioKitLZP 065 reaction tubes with capPreparation of the inoculation solutionAdd to reaction tube 1inoculation material (from LZC 555)1 level dosing spoon (blue)buffer solution (from LZC 555)10 mlMix thoroughly for 1 min and leave to settle for 20 min.Then pipette into reaction tube 2solution from reaction tube 10.2 mlchlorine-free tap water10 mland mix.Procedure I with cuvette test LCK 554Fill sample cuvette 1 with the sample or a diluted (1 : 2)sample, then analyse with procedure II (1. direct measurement).Pipette 0.2 ml of the diluted solution from reaction tube 2 intosample cuvette 2, then top up with sample or diluted (1 : 2)sample. Leave to stand for 5 days, then evaluate withprocedure II (2. measurement after 5 days).Calculation of the BOD5 contentSubtract 0.1 mg/l BOD5 from the displayed result.

LCK 554LASA 1 / plusEdition 98/04BOD5LCK 554LASA 10 / 20Edition 98/04BOD5EvaluationEvaluation1. Press ”Mode“ key and check program control number:–– : 402. Insert program filter 623 nm.3. Select test with ”Mode“ key.4. Insert sample cuvette 1 (direct measurement).Make a note of the displayed result (see table) ”A1“ resp.insert sample cuvette 2 (measurement after 5 days).Make a note of the displayed result (see table) ”A5“.1. Press any key.2. Check program control number: –– : 403. Select test with or key.4. Insert sample cuvette 1 (direct measurement).Make a note of the displayed result (see table) ”A1“ resp.insert sample cuvette 2 (measurement after 5 days).Make a note of the displayed result (see table) ”A5“.Calculation of the BOD5-concentrationA1 - A5 mg/l BOD5Diluted samples:The dilution factor must be taken into account when the BOD5 content iscalculated (measurement result x 2).ParameterBOD5DisplayBOD5 LCK 554Meas. range0.5 – 12 mg/lLCK 554LASA 30Edition 98/04BOD5Calculation of the BOD5-concentrationA1 - A5 mg/l BOD5Diluted samples:The dilution factor must be taken into account when the BOD5 content iscalculated (measurement result x 2).ParameterBOD5DisplayBOD5 LCK 554Meas. range0.5 – 12 mg/lLCK 554LP2WEdition 98/04BOD5EvaluationEvaluation1. Insert filter 605 nm.2. Select »Dr. Lange« mode.3. Select test number (see below).4. Control number must be 2.5. Insert sample cuvette 1 (direct measurement) andpress green key.Make a note of the displayed result (see table) ”A1“ resp.insert sample cuvette 2 (measurement after 5 days) andpress green key.Make a note of the displayed result (see table) ”A5“.1. Insert program filter 620 nm.2. Press ”Tests“ key until display (see below) appears.3. Control number must be 6.4. Insert blank-value cuvette (distilled water) andpress ”Null“ (zero) key.5. Insert sample cuvette 1 (direct measurement) andpress ”Ergebnis“ (result) key.Make a note of the displayed result (see table) ”A1“ resp.insert sample cuvette 2 (measurement after 5 days) andpress ”Ergebnis“ (result) key.Make a note of the displayed result (see table) ”A5“.Calculation of the BOD5-concentrationA1 - A5 mg/l BOD5Diluted samples:The dilution factor must be taken into account when the BOD5 content iscalculated (measurement result x 2).ParameterBOD5Test-No.554Meas. range0.5 – 12 mg/lCalculation of the BOD5-concentrationA1 - A5 mg/l BOD5Diluted samples:The dilution factor must be taken into account when the BOD5 content iscalculated (measurement result x 2).ParameterBOD5DisplayBOD5 LCK 554Meas. range0.5 – 12 mg/lAE 554 E / Druckfarbe blau / 2